Inventi Rapid: Pharm Biotech & Microbio

Inventi:prbm/18611/21

Microbiological Analysis of Vancomycin Resistant Enterococci

Vancomycin resistance enterococci particularly E. facials and E. faecium have emerged in nosocomial infections. The purpose of this study was to isolate enterococci from different clinical and environmental specimens, studying the susceptibility of the recovered isolates to vancomycin and some other antimicrobial, in the present study out of 277 collected clinical and environmental samples 153(55%) enterococcus isolates were recovered. Of the 226 clinical samples 146(64%) enterococcus isolates were recovered and of 51 environmental samples 7 (13.7%) enterococcus isolates were recovered. Susceptibility of the tested enterococcus isolates to vancomycin was determined by disc diffusion method. Out of 153 enterococcus isolates there were 18 (11, 7%) vancomycin resistant (VR). The MIC of all enterococcus isolates were also determined by agar dilution method using vancomycin concentrations ranging from 4-1024 µg/ml. According to NCCLS isolates of MIC ≥32 µg/ml are considered VR. Using agar dilution method there were only 11VR out of 153(7%) enterococcus isolates. However 18 VR isolates were detected using disc diffusion method which means that the resistance of 7 isolates of MIC <32 µg/ml were not detectable using agar dilution technique. In this study 11 VR enterococcus isolates that showed resistant either by disc diffusion or agar dilution were selected for further studies. These VR isolates were biochemically identified to the species level. The plasmid profiles of the selected 11 VRE isolates in addition to 17 vancomycin sensitive enterococcus (VSE) (10 VSE clinical isolates and 7 VSE environmental isolate) were determined using agarose gel electrophoresis....